Nowa metoda pozyskiwania tkanki mózgowej chorych na epilepsję do analiz omicznych z użyciem elektrod ze stereoelektroencefalografii klinicznej
A novel method for obtaining epileptic brain tissue for omic analyses using electrodes from a clinical stereoelectroencephalography study
W skrócie
[Preprint - wstępne wyniki] Naukowcy opracowali metodę zbierania próbek tkanki mózgowej przylegającej do elektrod używanych w leczeniu opornej na leki epilepsji. Z próbek tych wyekstrahowano RNA i proteiny, a analiza wykazała, że w obszarze originacji padaczki najsilniejsze są oznaki zapalenia, przy czym wyniki badań genów i białek się uzupełniały. Metoda ta pozwoli na głębsze zrozumienie molekularnych przyczyn epilepsji i może być przydatna do opracowania nowych sposobów leczenia.
Oryginalny abstract (angielski)
Abstract Purpose: Developing a valid and practical method for obtaining brain tissue samples for transcriptomic and proteomic analyses using tissue adhered to stereoelectroencephalography (SEEG) electrodes, to enable investigation of the molecular mechanisms underlying chronic epilepsy. Method: Brain tissue samples adhered to SEEG electrodes were collected from six patients with drug-resistant epilepsy and preprocessed in a hospital environment after electrode removal. RNA extraction was initiated immediately, and proteomics samples were snap-frozen until subsequent analysis. The samples were categorized into three groups according to their electrophysiological profile: epileptogenic zone, propagation zone, and least-involved zone. The omic findings between these zones and anatomical brain areas were compared. Results: High-quality RNA and protein samples were obtained from tissue adhered to SEEG electrodes. Neuron- and brain-specific gene expression patterns and proteins were identified. Signs of activation of inflammatory mechanisms were most pronounced in the epileptogenic zone. Transcriptomic and proteomic findings demonstrated concordance. Conclusion: SEEG electrodes are a useful source for obtaining brain tissue for molecular characterization of chronic epilepsy. This method will enable the identification of shared and distinct molecular mechanisms in patients with varying etiologies of epilepsy.