Gen KCNT1 kodujący kanał potasowy związany z epilepsją jest niezbędny do prawidłowego rozwoju komórek wielorzędnicowych u żaby Xenopus
Epilepsy-associated potassium channel KCNT1 is required for multiciliated cell specification in Xenopus
W skrócie
Badacze odkryli, że mutacje genu KCNT1 powodują nie tylko ciężką epilepsję, ale też problemy z oddychaniem i wadami serca u dzieci, co sugeruje, że gen ten jest ważny dla rozwoju wielu organów, a nie tylko mózgu. Przeprowadzili doświadczenia na żabach, które wykazały, że KCNT1 jest niezbędny do prawidłowego tworzenia się włosków (rzęsek) w komórkach, szczególnie w drogach oddechowych. Naukowcy znaleźli też możliwy sposób na łagodzenie skutków uszkodzenia tego genu poprzez oddziaływanie na inny kanał komórkowy o nazwie Piezo1, co może prowadzić do nowych sposobów leczenia pacjentów z mutacjami KCNT1.
Oryginalny abstract (angielski)
Pathogenic, presumed gain-of-function variants in the gene KCNT1, which encodes a sodium-activated potassium channel, cause a severe neurodevelopmental disorder with intractable epilepsy. In addition to seizures, affected individuals commonly present with severe respiratory issues and structural heart defects not commonly observed in other genetic pediatric epilepsies, suggesting additional developmental functions for KCNT1 in organs beyond the brain. Here we characterized the spectrum of clinical diagnoses present in a cohort of 46 individuals with genetic variants in KCNT1, ranging from 0 to 19 years of age, by medical record review. We documented the prevalence of diagnoses across organ systems, including dependence on assisted breathing, congenital structural heart defects, urinary dysfunction, and spine deformities, among others. Next, we explored the embryonic expression and function of KCNT1 in diploid frogs (Xenopus tropicalis) and observed expression in developing ciliated tissues such as the brain, heart, kidney, and epidermis. Embryonic perturbation of KCNT1 disrupted developmental signaling pathways and caused ciliogenesis defects in the mucociliary epidermis, a common model for the human airway. Loss of KCNT1 disrupted development of multiciliated cells, reminiscent of recent work on the ion channel Piezo1. Consistently, pharmacological inhibition of Piezo signaling enhanced the ciliogenesis phenotype observed following KCNT1 inhibition, while activation of Piezo1 activity partially rescued ciliogenesis in the context of KCNT1 inhibition. Finally, KCNT1 loss reduced expression of foxj1, a key transcriptional regulator of multiciliated cell specification, linking KCNT1 activity to the transcriptional programs underlying multiciliated cell formation and specification. Together, this work establishes that KCNT1 has embryonic functions in Xenopus beyond regulating neuronal activity, specifically in multiciliated cell development, and identifies an interaction with pharmacologically-tractable Piezo channels that may be productive for therapeutic efforts aimed at inhibiting KCNT1 activity.